Associate Prof. Hou Ximiao

　　　　Contact Information:

　　　　Mailing Address:

　　　　College of Life Sciences

　　　　Northwest A&F University

　　　　Yangling, Shaanxi 712100

　　　　P. R. China

　　　　Phone: (86) 29-87081664

　　　　Fax: (86) 29-87081664

　　　　E-mail: houximiao@nwsuaf.edu.cn

　　　　Office: South Campus, Scientific Research Building, 3310

　　　　Research Interests:

　　       Our research interest is using physical methods to answer fundamental biological questions at single molecule level, for example, what is the exact mechanism for a helicase to unwind duplex DNA; how polymerase works in the process of RNA transcription and DNA replication; how helicase and polymerase cooperate with each other during DNA metabolism. Currently, we are working on G-quadruplex DNA: How G-quadruplex folds and unfolds at single molecule level, the way G-quadruplex unfolds by various helicases, and the effects of G-quadruplex on the DNA, RNA polymerases. Basically, we use single fluorescence resonance energy transfer. Bulk assay including stopped-flow, fluorescence anisotropy and dynamic light scattering methods are also employed.

 

　　　　Education Experiences:

　　　　Institute of Physics, Chinese Academy of Sciences. Biophysics (major), Ph.D. 2009

　　　　Department of Physics, Beijing Normal University. Physics (major), B.S. 2004

　　　　Professional Experiences:

　　　　Associate Professor in College of Life Science, Norwest A&F University (2012-)

　　　　Postdoctoral fellow in College of Pharmacy, University of Michigan (2009-2012).

 

　　　　Publications:

　　　　Xi-Miao Hou, Yi-Ben Fu, Wen-Qiang Wu, Lei Wang, Fang-Yuan Teng, Ping Xie, Peng-Ye Wang, Xu-Guang Xi; (2017) Involvement of G-triplex and G-hairpin in the multi-pathway folding of human telomeric G-quadruplex. Nucleic Acids Research , https://doi.org/10.1093/nar/gkx766

　　　　Xi-Miao Hou, Xing-Hua Zhang, Kong-Ji Wei, Chao Ji, Shuo-Xing Dou, Wei-Chi Wang, Ming Li and Peng-Ye Wang (2009) Cisplatin induces loop structures and condensation of single DNA molecules. Nucleic Acids Research , 37, 1400-1410.

　　　　Bo Zhang, Wen-Qiang Wu, Na-Nv Liu, Xiao-Lei Duan, Ming Li, Shuo-Xing Dou and Xi-Miao Hou* and Xu-Guang Xi* (2016) G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction. Nucleic Acids Research , 44, 8385-8394.

　　　　Wen-Qiang Wu, Xi-Miao Hou*, Ming Li, Shuo-Xing Dou and Xu-Guang Xi*. (2015) BLM unfolds G-quadruplexes in different structural environments through different mechanisms. Nucleic Acids Research . 43(9), 4614-4626.

　　　　Wei Li^#, Xi-Miao Hou^#, Peng-Ye Wang, Xu-Guang Xi and Ming Li. (2013) Direct Measurement of Sequential Folding Pathway and Energy Landscape of Human Telomeric G-quadruplex Structures. Journal of American Chemical Society . 135:6423-6426. (并列第一作者)

　　　　Xi-Miao Hou, Wen-Qiang Wu, Xiao-Lei Duan, Na-Nv Liu, Hai-Hong Li, Jing Fu, Shuo-Xing Dou, Ming Li and Xu-Guang Xi. (2015) Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase. Biochemical Journal . 466:189-199.

　　　　Xi-Miao Hou and Wei Cheng. (2012). Detection of single fluorescent proteins inside eukaryotic cells using two-photon fluorescence. Biomedical Optics Express . 3: 340-353.

　　　　Xi-Miao Hou and Wei Cheng. (2011). Single-Molecule Detection Using Continuous-Wave Excitation of Two-Photon Fluorescence. Optics Letters . 36: 3185-3187.

　　　　Xi-Miao Hou, Wei Li, Shuo-Xing Dou, Ling-Yun Zhang, Wei-Chi Wang and Peng-Ye Wang. (2009) Formation of DNA toroids confined inside droplets absorbed onto mica surface. Physical Review E , 79, 051912.

　　　　Wen-Qiang Wu, Xi-Miao Hou, Bo Zhang, Philippe Fossé, Brigitte René, Olivier Mauffret,

　　　　Ming Li, Shuo-Xing Dou, and Xu-Guang Xi. Single-molecule studies reveal reciprocating of

　　　　WRN helicase core along ssDNA during DNA unwinding. Scientific Reports , 2017：43954.

　　　　Hai-Peng Ju, Yi-Zhou Wang, Jing You, Xi-Miao Hou, Xu-Guang Xi, Shuo-Xing Dou, Wei Li, and Peng-Ye Wang. (2016) Folding Kinetics of Single Human Telomeric G‑Quadruplex Affected by Cisplatin. ACS Omega , 1, 244−250

　　　　Wei Cheng, Xi-Miao Hou, Fang-Mao Ye. (2010) Use of tapered amplifier diode laser for biological-friendly high-resolution optical trapping[J]. Optics Letters, 35(17):2988-90.

　　　　Koh, B., Park, J. B., Xi-Miao Hou, Cheng, W. (2011). Comparative dispersion studies of single-walled carbon nanotubes in aqueous solution. Journal of Physical Chemistry B , 115(11), 2627-33.

　　　　Pang, Y., Song, H., Kim, J. H., Xi-Miao Hou, Cheng, W. (2014). Optical trapping of individual human immunodeficiency viruses in culture fluid reveals heterogeneity with single-molecule resolution. Nature Nanotechnology , 9(8), 624-30.

　　　　Yu-Dong Zhang, Shu-Xin Hu, Bing Yuan, Xi-Miao Hou, Ying Lu, Ming Li. (2009). Subnanometer-thick pbs film on organic substrate and its usage as buffer for homo- and heteroepitaxial overgrowth. Langmuir , 25(10), 5609-5613.

　　　　Xing-Hua Zhang, Xi-Miao Hou, Chao Ji, Ming Li and Peng-Ye Wang, (2009) Spermidine-Induced Two Dimensional DNA Condensation on Mica Surface: A Different Pathway from the Condensation in Solution”. Chinese Science Bulletin , 54, 2425-2433.