刘杰等《New Phytologist》2022年

论文题目：Sugar transporter TaSTP3 activation by TaWRKY19/61/82 enhances stripe rust susceptibility in wheat

论文作者：Baoyu Huai，Pu Yuan，Xiaoxuan Ma，Xiurui Zhang，Lihua Jiang，Peijing Zheng，Mohan Yao，Ziyu Chen，Liyang Chen，Qianhua Shen，Zhensheng Kang，Jie Liu

论文摘要：Sugar efflux from host plants is essential for pathogen survival and proliferation. Sugar transporter-mediated redistribution of host sugar contributes to the outcomes of plant-pathogen interactions. However, few studies have focused on how sugar translocation is strategically manipulated during host colonization. To elucidate this question, the wheat sugar transport protein (STP) TaSTP3 responding to Puccinia striiformis f. sp. tritici (Pst) infection was characterized for sugar transport properties in Saccharomyces cerevisiae and potential role during Pst infection by RNA interference and overexpression in wheat. In addition, the transcription factors regulating TaSTP3 expression was further determined. Our results showed that TaSTP3 is localized to the plasma membrane and functions as a sugar transporter of hexose and sucrose. TaSTP3 confers enhanced wheat susceptibility to Pst and overexpression of TaSTP3 resulted in increased sucrose accumulation and transcriptional suppression of defense-related genes. Furthermore, TaWRKY19, TaWRKY61 and TaWRKY82 were identified as positive transcriptional regulators of TaSTP3 expression. Our findings reveal that Pst-induced sugar transporter TaSTP3 is transcriptionally activated by TaWRKY19/61/82 and faci litates wheat susceptibility to stripe rust possibly through elevated sucrose concentration, and suggest TaSTP3 as a strong target for engineering wheat resistance to stripe rust.

寄主植物的糖分输出对病原菌的生存和增殖至关重要。糖转运子介导寄主糖分的重新分配对于植物与病原体相互具有重要影响。然而，很少有研究关注病菌侵染过程中寄主糖分转运是如何被策略性操纵的。为了阐明这一问题，本论文研究了响应条锈菌侵染的小麦糖运输蛋白(STP) TaSTP3的糖转运特性，以及通过RNA干扰和过表达技术明确其在小麦条锈菌侵染过程中的作用。此外，我们还进一步确定了调控TaSTP3表达的转录因子。我们的研究结果表明，TaSTP3定位于质膜，作为己糖和蔗糖的糖转运体。TaSTP3增强了小麦对条锈菌的敏感性，过表达TaSTP3导致了蔗糖积累增加和防御相关基因的转录抑制。此外，TaWRKY19、TaWRKY61和TaWRKY82被鉴定为TaSTP3表达的正向转录调控因子。我们的研究结果表明，条锈菌诱导的糖转运蛋白TaSTP3可被TaWRKY19/61/82协同转录激活，并可能通过提高蔗糖浓度来促进小麦对条锈菌的感病性，表明TaSTP3可以作为小麦抗条锈病遗传改良的一个有效靶点。

论文链接：https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.18331