麻鹏达、董娟娥等《Horticulture Research》2023年

论文题目：The SmMYB36-SmERF6/SmERF115 module regulates the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza hairy roots

论文作者：Qi Li#, Xin Fang#, Ying Zhao#, Ruizhi Cao, Juane Dong* and Pengda Ma*

论文摘要：Tanshinone and phenolic acids are the most important active substances of Salvia miltiorrhiza, and the insight into their transcriptional regulatory mechanisms is an essential process to increase their content in vivo. SmMYB36 has been found to have important regulatory functions in the synthesis of tanshinone and phenolic acid; paradoxically, its mechanism of action in S. miltiorrhiza is not clear. Here, we demonstrated that SmMYB36 functions as a promoter of tanshinones accumulation and a suppressor of phenolic acids through the generation of SmMYB36 overexpressed and chimeric SmMYB36-SRDX (EAR repressive domain) repressor hairy roots in combination with transcriptomic-metabolomic analysis. SmMYB36 directly down-regulate the key enzyme gene of primary metabolism, SmGAPC, up-regulate the tanshinones biosynthesis branch genes SmDXS2, SmGGPPS1, SmCPS1 and down-regulate the phenolic acids biosynthesis branch enzyme gene, SmRAS. Meanwhile, SmERF6, a positive regulator of tanshinone synthesis activating SmCPS1, was up-regulated and SmERF115, a positive regulator of phenolic acid biosynthesis activating SmRAS, was down-regulated. Furthermore, the seven acidic amino acids at the C-terminus of SmMYB36 are required for both self-activating domain and activation of target gene expression. As a consequence, this study contributes to reveal the potential relevance of transcription factors synergistically regulating the biosynthesis of tanshinone and phenolic acid.

丹参酮和丹酚酸是丹参最重要的活性物质，深入了解其转录调控机制是提高含量的一个重要过程。SmMYB36已被发现在丹参酮和酚酸的合成中具有重要的调节功能，但它在丹参中的作用机制还不清楚。在此，我们通过生成SmMYB36过表达和SmMYB36-SRDX（EAR抑制域）抑制的毛状根，结合转录组-代谢组分析，证明了SmMYB36作为丹参酮积累的正调控蛋白和丹酚酸生物合成的抑制蛋白的功能。SmMYB36直接下调初级代谢的关键酶基因SmGAPC，上调丹参酮生物合成支路酶基因SmDXS2、SmGGPPS1、SmCPS1，下调丹酚酸类生物合成分支酶基因SmRAS。同时，激活SmCPS1的丹参酮合成正调控因子SmERF6被上调，激活SmRAS的酚酸生物合成正调控因子SmERF115被下调。此外，SmMYB36的C端七个酸性氨基酸是自激活域和激活靶基因表达所必需的。因此，本研究有助于揭示转录因子协同调控丹参酮和酚酸的生物合成的潜在相关性。

论文链接：https://academic.oup.com/hr/article/10/1/uhac238/6775198