董娟娥等《Industrial Crops & Products》2022年
论文题目：Sulfonated, quaternized, and chlorogenic acid composited sodium alginate hydrogels/Eucommia ulmoidesrubber films as in vitro antibacterial wound dressings for accelerating wound healing
论文作者：Caifeng Guo , Ziyun Zhou , Siqi Zhang , Pai Peng , Jingling Liu , Yonggang Yan ,Juane Dong
论文摘要：
It is important to improve antibacterial properties and maintain desirable humidity of wound dressings. Herein, novel antibacterial wound dressings were prepared. Sulfonated SA, quaternized SA, and chlorogenic acid composited SA hydrogels were used and coated onEucommia ulmoidesrubber (EUR) films. These hydrogels were characterized by FTIR, SEM, zeta potential, swelling behavior and rheology. Moreover, the biocompatibility of the hydrogels was studied by fibroblast cells (L929). Then, the mechanical property and water vapor permeability of wound dressings were tested. Meanwhile, the drug release behavior of the wound dressings was determined by the Korsmeyer-Peppas model and their antibacterial effects onE. coliandS. aureuswere studied. Finally, wound healing performance was evaluated by mouse full-thickness wounds model. After 11 days, the wound healing rates in the treatment groups can reach more than 90%, in particular, CGA-SA/EUR group had the highest healing rate of 98%. The results from histopathological observation and immunohistochemical staining showed these wound dressings could effectively promote the wound regeneration and repairing, indicating the wound dressings can be potentially used for accelerating wound healing.
提高创面敷料的抗菌性能和保持适宜的湿度非常重要。本研究制备了新型抗菌创面敷料。采用磺化、季铵化和绿原酸复合海藻酸钠水凝胶涂敷在杜仲橡胶(EUR)薄膜上。用FTIR、SEM、zeta电位、溶胀行为和流变性等对这些水凝胶进行了表征。用成纤维细胞(L929)研究了水凝胶的生物相容性。对创面敷料的力学性能和透气性进行了测试。同时，采用Korsmeyer-Peppas模型测定创面敷料的药物释放行为，研究其对大肠杆菌和金黄色葡萄球菌的抗菌作用。最后用小鼠全层创面模型评价创面愈合性能。结果发现，11 d后，各治疗组创面愈合率均可达90%以上，其中CGA-SA/EUR组愈合率最高，可达98%。组织病理学观察和免疫组化染色结果表明，这些创面敷料能有效促进创面再生和修复，提示创面敷料有加速创面愈合的潜力。