侯锡苗
一、 学习及工作经历
2021.1- 生命科学学院 教授
2012.4-2020.12 生命科学学院 副教授
2009.6-2012.2 美国密歇根大学 药学院 博士后
2004.9-2009.5 中科院物理研究所 软物质物理实验室 凝聚态物理学 博士
2000.9-2004.7 北京师范大学 物理学系 物理学 学士
二、 主要研究方向
主要研究领域为单分子生物学与结构生物学。自2012年入职至今，主持建立了单分子研究平台，在国内率先将单分子荧光成像应用于蛋白质与核酸互作机理的研究。现阶段工作主要围绕“染色质结构的动态调控”展开，具体针对核酸G-四链体的结构与功能进行研究。包括：1. 四链核酸DNA、RNA的形成机制与变化规律；2. 蛋白质对G4结构进行调控的分子机制；3. G4结构与癌基因表达调控；4. G4结构与病毒转录复制。
三、 主讲课程
《基础生物化学》、《生物大分子结构解析》、《单分子行为研究方法》、《结构生物学》
四、 主要研究成果
第一/通讯作者论文
1. Yi-Ran Wang; Ting-Ting Guo; Ya-Ting Zheng; Chang-Wei Lai; Bo Sun; Xu-Guang Xi; Xi-Miao Hou*. Replication protein A plays multifaceted roles complementary to specialized helicases in processing G-quadruplex DNA. iScience , 2021, doi.org/10.1016/j.isci.2021.102493.
2. Gao Bo, Xi-Miao Hou*. Opposite Effects of Potassium Ions on the Thermal Stability of i-Motif DNA in Different Buffer Systems. ACS Omega , 6: 8976 (2021)
3. Fang-Yuan Teng, Ting-Ting Wang, Hai-Lei Guo, Ben-Ge Xin, Bo Sun, Shou-Xing Dou, Xu-Guang Xi*, Xi-Miao Hou*. The HRDC domain oppositely modulates the unwinding activity of E. coli RecQ helicase on duplex DNA and G-quadruplex. Journal of Biological Chemistry. 295:17646 (2020).
4. Qing-Man Wang, Yan-Tao Yang, Yi-Ran Wang, Bo Gao, Xu-Guang Xi, and Xi-Miao Hou*. Human Replication protein A induces dynamic changes in single-stranded DNA and RNA structures. Journal of Biological Chemistry . 294:13915 (2019).
5. Lei Wang, Qing-Man Wang, Yi-Ran Wang, Xu-Guang Xi, and Xi-Miao Hou*. DNA-unwinding activity of Saccharomyces cerevisiae Pif1 is modulated by thermal stability, folding conformation, and loop lengths of G-quadruplex DNA. Journal of Biological Chemistry . 293:18504 (2018).
6. Xi-Miao Hou#*, Yi-Ben Fu#, Wen-Qiang Wu, Lei Wang, Fang-Yuan Teng, Ping Xie, Peng-Ye Wang, Xu-Guang Xi*. Involvement of G-triplex and G-hairpin in the multi-pathway folding of human telomeric G-quadruplex. Nucleic Acids Research , 45:11401 (2017).
7. Bo Zhang, Wen-Qiang Wu, Na-Nv Liu, Xiao-Lei Duan, Ming Li, Shuo-Xing Dou and Xi-Miao Hou* and Xu-Guang Xi*. G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction. Nucleic Acids Research , 44: 8385 (2016).
8. Wen-Qiang Wu, Xi-Miao Hou*, Ming Li, Shuo-Xing Dou and Xu-Guang Xi*. BLM unfolds G-quadruplexes in different structural environments through different mechanisms. Nucleic Acids Research . 43: 4614 (2015).
9. Xi-Miao Hou#, Wen-Qiang Wu#, Xiao-Lei Duan, Na-Nv Liu, Hai-Hong Li, Jing Fu, Shuo-Xing Dou*, Ming Li and Xu-Guang Xi*. Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase. Biochemical Journal . 466:189-199 (2015).
10. Wei Li#, Xi-Miao Hou#, Peng-Ye Wang, Xu-Guang Xi and Ming Li*. Direct Measurement of Sequential Folding Pathway and Energy Landscape of Human Telomeric G-quadruplex Structures. Journal of American Chemical Society . 135:6423-6426 (2013).
11. YZ Wang#，XM Hou#，HP Ju，X Xiao，XG Xi，SX Dou，PY Wang，W Li*. Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers. Chinese Physics B . 2018, 27:541
12. X. Hou, M. C. DeSantis, C. Tian, and W. Cheng*. (2016). Optical Manipulation of a Single Human Virus for Study of Viral-Cell Interactions. Proc. of SPIE 9922: 992212. doi: 10.1117/12.2239051
13. Xi-Miao Hou and Wei Cheng*. (2012). Optical Tweezers. In: Roberts GC. Encyclopedia of Biophysics . Heidelberg, Springer.
14. Xi-Miao Hou and Wei Cheng*. (2012). Detection of single fluorescent proteins inside eukaryotic cells using two-photon fluorescence. Biomedical Optics Express . 3: 340-353.
15. Xi-Miao Hou and Wei Cheng*. (2011). Single-Molecule Detection Using Continuous-Wave Excitation of Two-Photon Fluorescence. Optics Letters . 36: 3185-3187.
16. Xi-Miao Hou, Wei Li, Shuo-Xing Dou, Ling-Yun Zhang, Wei-Chi Wang and Peng-Ye Wang*. (2009) Formation of DNA toroids confined inside droplets absorbed onto mica surface. Physical Review E , 79, 051912.
17. Xi-Miao Hou, Xing-Hua Zhang, Kong-Ji Wei, Chao Ji, Shuo-Xing Dou, Wei-Chi Wang, Ming Li and Peng-Ye Wang* (2009) Cisplatin induces loop structures and condensation of single DNA molecules. Nucleic Acids Research , 37, 1400-1410.
共同作者论文
18. Ye, S.S., Chen, Z.T., Zhang, X., Li, F.F., Guo, L.J., Hou, X.M., Wu, W.Q., Wang, J., Liu, C., Zheng, K., and Sun, B.*Proximal single-stranded RNA destabilizes human telomerase RNA G-quadruplex and induces its distinct conformers. The Journal of Physical Chemistry Letters , 2021, 12:3361
19. Yang-Xue Dai, Wei-Fei Chen , Na-Nv Liu, Fang-Yuan Teng , Hai-Lei Guo, Xi-Miao Hou, Shuo-Xing Dou, Stephane Rety , Xu-Guang Xi*. Structural and functional studies of SF1B Pif1 from Thermus oshimai reveal dimerization-induced helicase inhibition. Nucleic Acids Res , 2021, 49:4129.
20. Qin Z, Bi L, Hou XM, Zhang S, Zhang X, Lu Y, Li M, Modesti M, Xi XG, Sun B*. Human RPA activates BLM's bidirectional DNA unwinding from a nick. Elife , 2020, 9:e54098
21. Siqi Zhang, Qian Zhang, Xi-Miao Hou, Lijuan Guo, Fangzhu Wang, Lulu Bi, Xia Zhang, Hai-Hong Li, Fengcai Wen, Xu-Guang Xi, Xingxu Huang, Bin Shen, Bo Sun*. Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation. EMBO Reports, 2020, 21(10):e50184
22. Fang-Yuan Teng, Zong-Zhe Jiang, Ling-Yun Huang, Man Guo , Feng Chen , Xi-Miao Hou, Xu-Guang Xi, Yong Xu*. A Toolbox for Site-Specific Labeling of RecQ Helicase With a Single Fluorophore Used in the Single-Molecule Assay. Front Mol Biosci . 2020, 7:586450
23. Wei-Fei Chen , Stephane Rety , Hai-Lei Guo , Yang-Xue Dai , Wen-Qiang Wu , Na-Nv Liu , Daniel Auguin , Qian-Wen Liu, Xi-Miao Hou, Shuo-Xing Dou , Xu-Guang Xi*. Molecular Mechanistic Insights into Drosophila DHX36-Mediated G-Quadruplex Unfolding: A Structure-Based Model. Structure , 2018, 26(3):403-415
24. Wen-Qiang Wu, Xi-Miao Hou, Bo Zhang, Philippe Fossé, Brigitte René, Olivier Mauffret,
Ming Li, Shuo-Xing Dou, and Xu-Guang Xi. Single-molecule studies reveal reciprocating of
WRN helicase core along ssDNA during DNA unwinding. Scientific Reports , 2017：43954.
25. Fang-Yuan Teng, Xi-Miao Hou, San-Hong Fan , Stephane Rety , Shuo-Xing Dou, Xu-Guang Xi*. Escherichia coli DNA polymerase I can disrupt G-quadruplex structures during DNA replication. FEBS J . , 2017, 284(23):4051
26. Hai-Peng Ju, Yi-Zhou Wang, Jing You, Xi-Miao Hou, Xu-Guang Xi, Shuo-Xing Dou, Wei Li, and Peng-Ye Wang. (2016) Folding Kinetics of Single Human Telomeric G‑Quadruplex Affected by Cisplatin. ACS Omega , 1, 244−250
27. Pang, Y., Song, H., Kim, J. H., Xi-Miao Hou, Cheng, W. (2014). Optical trapping of individual human immunodeficiency viruses in culture fluid reveals heterogeneity with single-molecule resolution. Nature Nanotechnology , 9(8), 624-30.
28. Koh, B., Park, J. B., Xi-Miao Hou, Cheng, W. (2011). Comparative dispersion studies of single-walled carbon nanotubes in aqueous solution. Journal of Physical Chemistry B , 115(11), 2627-33.
29. Wei Cheng, Xi-Miao Hou, Fang-Mao Ye. (2010) Use of tapered amplifier diode laser for biological-friendly high-resolution optical trapping[J]. Optics Letters , 35(17):2988-90.
30. Yu-Dong Zhang, Shu-Xin Hu, Bing Yuan, Xi-Miao Hou, Ying Lu, Ming Li. (2009). Subnanometer-thick pbs film on organic substrate and its usage as buffer for homo- and heteroepitaxial overgrowth. Langmuir , 25(10), 5609-5613.
31. Xing-Hua Zhang, Xi-Miao Hou, Chao Ji, Ming Li and Peng-Ye Wang, (2009) Spermidine-Induced Two Dimensional DNA Condensation on Mica Surface: A Different Pathway from the Condensation in Solution”. Chinese Science Bulletin , 54, 2425-2433.
五、 主持科研项目
1. 国家自然科学基金面上项目 特异解旋酶在G-四链体RNA介导的原癌基因表达调控中的作用及机制， 2021-2024， 58 万
2. 国家自然科学基金青年项目 用单分子方法研究G-四链体DNA结构稳定性和解旋机制，2014-2016，30万元
3. 陕西省自然科学基础研究计划 特异解旋酶对G-四链体RNA进行调控的结构基础与分子作用机制，2020-2021，3万元
六、 联系方式
通讯地址：陕西省杨凌示范区 南校区科研楼3310
邮编：712100
Email： houximiao@nwsuaf.edu.cn