陈红英

       陈红英

女，博士 , 教授

联系方式

单位： 生命学院

地址： 陕西杨凌西农路22号，712100

电子邮件：chenhy@nwsuaf.edu.cn

1988-1992年，中国农业大学动物生理学与生物化学专业本科；1992-1998年，中国农业大学动物生理学与生物化学专业硕博连读；1998-2000年，中国科学院发育生物学研究所，博士后；2000-2009年，英国雷丁大学（University of Reading），Research fellow。2009年9月到 生命科学学院工作，入选2009年教育部新世纪优秀人才支持计划。

研究方向

动物病毒-宿主互作关系，病毒蛋白的结构功能，杆状病毒表达系统

发表论文

发表科研论文40余篇。代表性论文：

1.Xiaoyue Zhang, Kaixia Zhao, Lan Lan, Na Shi,| Hao Nan, Yanan Shi, Xiaodong Xu*, Hongying Chen*. Improvement of protein production by engineering a novel antiapoptotic baculovirus vector to suppress the expression of Sf-caspase-1 and Tn-caspase-1. Biotechnol Bioeng. 2021;May 15. doi: 10.1002/bit.27807

2.Yanfei Jiang, Hao Nan, Na Shi, Wenfang Hao, Juane Dong*, Hongying Chen*. Chlorogenic acid inhibits proliferation in human hepatoma cells by suppressing noncanonical NF-κB signaling pathway and triggering mitochondrial apoptosis. Molecular Biology Reports. 2021 Mar; 48(3):2351-2364. doi: 10.1007/s11033-021-06267-3.

3.Cao Z, Liu S, Nan H, Zhao K, Xu X, Wang G, Ji H, Chen H*. Immersion immunization with recombinant baculoviruses displaying cyprinid herpesvirus 2 membrane proteins induced protective immunity in gibel carp. Fish Shellfish Immunol. 2019 Aug 14;93:879-887. doi: 10.1016/j.fsi.2019.08.036

4.Wang L, Zhang L, Huang B, Li K, Hou G, Zhao Q, Wu C, Nan Y, Du T, Mu Y, Lan J, Chen H*, Zhou EM*. A Nanobody Targeting Viral Non-Structural Protein 9 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication. J Virol. 2019 Feb 5, 93(4): e01888~18. doi: 10.1128/JVI.01888-18

5.Nan H, Lan J, Tian M, Dong S,Tian J, Liu L, Xu X and Chen H* (2018) The Network of Interactions Among Porcine Reproductive and Respiratory Syndrome Virus Non-structural Proteins. Front. Microbiol. 9:970.

6. Xiaoyue Zhang, Keyan Xu, Yanmei Ou, Xiaodong Xu* and Hongying Chen* Development of a baculovirus vector carrying a small hairpin RNA for suppression of sf-caspase-1 expression and improvement of recombinant protein production. BMC Biotechnology (2018) 18:24

7. Chen J, Xu X, Tao H, Li Y, Nan H, Wang Y, Tian M, Chen H*. (2017) Structural Analysis of Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 7α (NSP7α) and Identification of its Interaction with NSP9. Front. Microbiol, 8:853.

8. Chen J, Xu X, Tao H, Wang Y, Chen H*. (2016) Chemical shift assignments of nsp7α from porcine reproductive and respiratory syndrome virus. Biomol NMR Assign, 10(2):391-394.

9. Dong S, Liu L, Wu W, Armstrong SD, Xia D, Nan H, Hiscox JA, Chen H* Determination of the interactome of non-structural protein12 from highly pathogenic porcine reproductive and respiratory syndrome virus with host cellular proteins using high throughput proteomics and identification of HSP70 as a cellular factor for virus replication. Journal of Proteomics 146: 58–69, 2016.

10. Liu L, Tian J, Nan H, Tian M, Li Y, Xu X, Huang B, Zhou E, Hiscox JA, Chen H*. Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Interacts with Nsp9 and Cellular DHX9 to Regulate Viral RNA Synthesis. J Virol. 2016, 90:5384–5398.

11. Xu X*, Zhou X, Nan H, Zhao Y, Bai Y, Ou Y, H Chen*. Aggregation of AcMNPV LEF-10 and Its Impact on Viral Late Gene Expression. PLoS ONE 11(5): e0154835, 2016.

12. Liu L, Lear Z, Hughes DJ, Wu W, Zhou E, Whitehouse A, Chen H*, Hiscox JA* Resolution of the cellular proteome of the nucleocapsid protein from a highly pathogenic isolate of porcine reproductive and respiratory syndrome virus identifies PARP-1 as a cellular target whose interaction is critical for virus biology. Veterinary Microbiology 2015, 176(1-2): 109-119.

13. Y Wang, Y Mao, X Xu, S Tao*, H Chen* Codon Usage in Signal Sequences Affects Protein Expression and Secretion Using Baculovirus/Insect Cell Expression System. PLoS ONE 10(12): e0145887, 2015.

14. Fang W, Peng K, Tian J, Xu X, Zhou E, Chen H* Immune response to Fc tagged GP5 glycoproteins of porcine reproductive and respiratory syndrome virus. Viral Immunology 2014, 27 (7): 343–349.

15. Liu B, Wang R, Wu F, Xu X, Chen H* Rapid production of HIV-1 neutralizing antibodies in baculovirus infected insect cells. Protein Expression and Purification 2014, 99：87-93.

16. Xu X, Chen Y, Zhao Y, Liu X, Dong B, Jones IM, Chen H* Baculovirus superinfection: A probable restriction factor on the surface display of proteins for library screening. PLoS ONE 8(1): e54631, 2013.

17. Chen H, Xu X, Lin HH, Chen SH, Forsman A, Chapman MA, Jones IM*. Mapping the immune response to the outer domain of a human immunodeficiency virus-1 clade C gp120. J. Gen. Virol. 2008 Oct;89(Pt 10):2597-2604.

18. Chen H, Xu X, Jones IM*. Immunogenicity of the outer domain of a HIV-1 clade C gp120. Retrovirology 2007; 4: 33.

19. Chen H, Xu X, Bishop A, Jones IM*. Reintroduction of the 2G12 epitope in an HIV-1 clade C gp120. AIDS. 2005 May 20;19(8):833-5.

20. Chen H, Gill A, Dove BK, Emmett SR, Kemp CF, Ritchie MA, Dee M, Hiscox JA*. Mass spectroscopic characterization of the coronavirus infectious bronchitis virus nucleoprotein and elucidation of the role of phosphorylation in RNA binding by using surface plasmon resonance. J Virol. 2005 Jan;79(2):1164-79.

21. Chen H, Coote B, Attree S, Hiscox JA*. Evaluation of a nucleoprotein-based enzyme-linked immunosorbent assay for the detection of antibodies against infectious bronchitis virus. Avian Pathol. 2003 Oct;32(5):519-26.

22. Chen H, Wurm T, Britton P, Brooks G, Hiscox JA*. Interaction of the coronavirus nucleoprotein with nucleolar antigens and the host cell. J Virol. 2002 May;76(10):5233-50.

23. Chen HY, Zhou Q, Zhang MF, and Giambrone JJ*. Sequence Analysis of the VP2 Hypervariable Region of Nine Infectious Bursal Disease Virus Isolates from Mainland China. Avian Diseases 42:762-769, 1998.

专利

陈红英，许晓东，杨雄 一种识别HIV-1受体结合表位的可溶性单链抗体的制备方法，专利号：ZL 2013 1 0088900.1。

一种新型冠状病毒表达载体，申请号：201910650551.5

一种展示cyhv-2膜蛋白的重组杆状病毒及其制备方法和应用，申请号：201910724103.5

一种展示cyhv-2膜蛋白的重组杆状病毒及其制备方法和应用，申请号：201910724104.X