麻鹏达、董娟娥等《Horticulture Research》2022年

论文题目：Functional pleiotropism, diversity, and redundancy of Salvia miltiorrhiza Bunge JAZ family proteins in jasmonate-induced tanshinone and phenolic acid biosynthesis.

论文作者：Pengda Ma, Tianlin Pei, Bingbing Lv, Mei Wang, Juane Dong, Zongsuo Liang

论文摘要：Jasmonate (JA) signaling regulates plant growth and development, biotic and abiotic stress tolerance, and primary and secondary metabolism biosynthesis. It is extensively modulated by JA-ZIM-domain (JAZ) family genes. In previous work, we obtained nineSmJAZgenes ofSalvia miltiorrhizaand proved that SmJAZ8 was the core repressor of JA-induced tanshinone and phenolic acid biosynthesis. Here, we demonstrate thatSmJAZ3andSmJAZ4act as repressors of JA-induced biosynthesis of tanshinones and salvianolic acid B (SalB). This suggests thatSmJAZ3/4are functionally redundant in tanshinone and Sal B biosynthesis.SmJAZ1/2/5/6/9are activators of JA-induced tanshinone biosynthesis and repressors of JA-induced SalB biosynthesis. This demonstrates the redundancy and diversity ofSmJAZ1/2/5/6/9functions. Besides,SmJAZ10inhibited JA-induced SalB synthesis, but had no effect on the synthesis of tanshinone. Two-hybrid screening (Y2H) showed that SmJAZs formed homologous or heterogeneous dimers. Y2H and firefly luciferase complementation imaging (LCI) assays revealed that SmJAZs also formed a complex regulatory network with SmMYC2a, SmMYC2b, SmMYB39, and SmPAP1. Quantitative reverse transcription-PCR (qRT-PCR) indicated thatSmJAZsregulated each other at the transcriptional level. Herein, we prove thatSmJAZs have functional pleiotropism, diversity, and redundancy in JA-induced tanshinone and phenolic acid biosynthesis. This study provides an important clue for further understanding the inherent biological significance and molecular mechanisms of theJAZfamily as the gene number increases during plant evolution.

茉莉酸（JA）信号调节植物的生长和发育、生物和非生物胁迫的耐受性以及初级和次级代谢的生物合成。JA信号被JA-ZIM结构域（JAZ）家族基因广泛地调控。在以前的工作中，我们克隆获得了丹参的9个SmJAZ基因，并证明SmJAZ8是JA诱导的丹参酮和酚酸生物合成的核心抑制因子。在此，我们证明SmJAZ3和SmJAZ4作为JA诱导的丹参酮和丹酚酸B（SalB）生物合成的抑制因子发挥作用。这表明SmJAZ3/4对丹参酮和SalB的生物合成有冗余的功能。SmJAZ1/2/5/6/9是JA诱导的丹参酮生物合成的激活因子和JA诱导的SalB生物合成的抑制因子。这表明SmJAZ1/2/5/6/9功能的冗余性和多样性。此外，SmJAZ10只抑制JA诱导的SalB的合成，但对丹参酮的合成没有影响。酵母双杂交（Y2H）实验表明，SmJAZs可形成同源或异源二聚体。Y2H和萤火虫荧光素酶互补成像（LCI）实验显示，SmJAZs还与SmMYC2a、SmMYC2b、SmMYB39和SMPAP1形成复杂的调控网络。实时荧光定量PCR（qRT-PCR）表明，SmJAZs在转录水平上相互调节。在此，我们证明了SmJAZs在JA诱导的丹参酮和酚酸生物合成中具有功能上的多效性、多样性和冗余性。这项研究为进一步揭示植物进化过程中JAZ家族基因内在生物学意义和分子机制提供了重要线索。

文章链接：https://academic.oup.com/hr/article/doi/10.1093/hr/uhac166/6649806